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#TestingMethods2020

Transport media that inactivates the virus

20 Ideas
11 Votes
209 Comments
42 Subscribers

In order to increase laboratory throughput we are looking at ways to minimise processes including the need to handle test samples in Category 2+/3 facilities. We are looking for transport media solutions that inactivate the virus reliably or do not add significant steps to the laboratory process or impact on viral detection.

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C: Further assessment

3G-202 virus sample preservation solution kits

Goldlane Ltd acts on behalf of NKY Medical Holdings (a company listed on the Shenzhen stock exchange) and their subsidiary 3G Biotech. Our virus specimen preservation solution kit provides for reliable and high quality RNA preservation and safe transportation. Samples taken by nasal or throat swabs can be safely transported at ambient temperature with minimal specialist equipment. The sample preservation solution stabilises the nucleic acid to be tested, improves the concentration of the...

10
E: A connection is helpful

PrimeStore MTM

PrimeStore® MTM's pathogen inactivation and nucleic acid stabilization characteristics make it the ideal solution for transporting samples that potentially contain dangerous infectious agents for molecular testing and Next Generation Sequencing. PrimeStore® MTM is optimized to inactivate/kill infectious biological pathogens, including influenza, mycobacterium tuberculosis, gram positive/negative bacteria, and viruses.  It disrupts/lyses lipid membranes and inactivates nucleases....

14
Closed
A: Not for current challenges

Microwaving the samples in any media compatible with dowstream applications

Microwave irradiation (in regular microwave ovens) has proved to be an efficient, cheap and convenient way of viral inactivation, allowing subsequent detection by RT-PCR or immunological methods. The media of use could be any compatible media (PBS, Tris-HCL, Hepes...) with subsequent applications.

4
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D2

Covid-19 testing swab with virus transport media kit

We offer testing kits including flocked swabs (for oral or NP), with virus transport media. 

5
Closed
A: Not for current challenges

CyMol

CyMol (Copan Italia, Brescia Italy) would seem to be a validated transport medium for samples destined for PCR/RT-PCR. See: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3122718/

4
Closed
A: Not for current challenges

Compatible Media to inactivate virus and subsequent pipeline structure

Use of standard detergent based lysis buffer compatible with RNA isolation kits. It is important to use RNA isolation kits in 96 ( or more) well format. I am sure it is easy to set up a 96 well plate RNA extraction assay connected to vacuum manifold. We do use similar pipeline for high throughput protein extraction for Mass Spectrometry based proteomics. 

4
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E: A connection is helpful

Virus Sampling Kits - Approved by NHS Scotland

We are able to supply a high quality swab and preservative / medium kit for the collection of samples. Swabs can be used for either nasopharyngeal or oropharyngeal specimen collection.  

13
A: Not for current challenges

Use Michels media to transport virus

I have used Michels media for solid tissue samples in histology to preserve skin samples safely. I have no idea about swab samples if its even practical but i know it fixes tissues in a different way to formalin is much safer for handling and that PCR works afterwards.   

6
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B: One to watch
Additional information

vivoPHIX™ RNA stabilisation and virus inactivation collection reagent and tube

RNAssist is a Cambridge, UK based technology development company focused on RNA stabilisation, pathogen inactivation and cell fixation, our lead product vivo PHIX™ has been evaluated by 240 researchers including at the University of Cambridge and Oxford, NIBSC, Pasteur, Sanger, CDC and MIT.  Offer; A sample collection tube for swabs or saliva, containing  vivo PHIX™ virus inactivation and viral RNA stabilisation reagent. 

22
Closed
A: Not for current challenges

Trizol or equivalent reagent - stable, well tested and good for downstream column prep.

Place samples in Trizol reagent for safe storage of RNA and complete inactivation of virus.

4
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