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Fast and scalable extraction protocol from AusDx
Have you validated this method, if so, how and what were the results of the validation?
The method so far was validated in-house on modeled CoVid-19, RSV and FluA samples against full extraction kit (MT-Prep, AusDiagnostics). The fast extraction has shown the delay of take off (Ct) in the range between 1 to 2.5 cycles. All modeled low positive samples were detected though. We also did reproducibility study between two types of magnetic racks and two operators. Variation of take off values in case of extraction of the same sample was +/-1 cycle
How quickly could this be deployed and what are the dependencies?
First trial batches planned in production in one week. We need 2-3 weeks to fill us the stock of raw materials and prepare production for a bigger scale.
What is the likely production volume?
Initial production capacity estimated at the level of 10,000 tests per day with potential rise to 30,000 tests per day (Sydney manufacturing site). It is also possible and planned to make a technology transfer to our UK manufacturing site and further increase the production scale.
What are the risks and barriers to using this at scale?
Supply of raw material is still limiting the production abilities the most. supply of raw materials for the production scale listed above has been secured through contracts. We are working on possible increase of supply
Who are you already partnering with on this?
A few Australia labs will start validations in a week
Sue Hill 10 months ago
Really interesting. What is the potential throughput within the laboratory setting given the move to a manual process from automated ?
Rob King 10 months ago
Status label added: D
Kyle Beacham 10 months ago
The idea has been progressed to the next milestone.
Bev Matthews 10 months ago
Status label added: D1
Status label removed: D
Tom Jordan 10 months ago
Thank you for sharing this protocol with us Dmitry. I am pleased to let you know that we have shared your suggestion with the diagnostics community via a weekly Testing Methods bulletin, to encourage labs to test this and scale up. The bulletin directs readers to this page to consider the protocol in detail.
As one of the earlier proposals we reviewed, your suggestion went out in issue 1 on the 16 April. You can access this from the COVID-19 resources hub on the RCPath website: https://www.rcpath.org/profession/coronavirus-resource-hub.html
Kind regards,
Tom Jordan
On behalf of the Testing Methods team
Tom Jordan 10 months ago
The idea has been progressed to the next milestone.
Dmitry Kuevda 10 months ago
Current status update: the method was widely tested on manual extraction protocols. Using of a deep well plate and a 8-channel pipette allows to extract 24 samples in less than 15 minutes. We also successfully programmed and tested 96-channel electronic pipette from Integra. The protocol time for 96 extractions lasts 15-20 min. The protocol runs at room temperature. The plate of extracts with magnetic beads could go straight to the robot for automatic liquid handling without reformatting (our magnet is compatible with standard SBS positions of most liquid handling robots)
Charlotte Cookson 9 months ago
Hi Dmitry, we are trying to make contact to see if you would like to join a conversation we are planning for next week. Charlotte
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Charlotte Cookson 9 months ago
Status label added: Requires more information
Bev Matthews 8 months ago
Status label added: D3: Catalogue of Resources
Status label removed: More information requested