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HEAT INACTIVATION, NO EXTRACTION NEEDED

D1: Path Lab Bulletin
D3: Catalogue of Resources

Title: An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.
Authors: Anna S. Fomsgaard and Maiken W. Rosenstierne
Affiliations:  Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark
Corresponding author: Maiken W. Rosenstierne
Corresponding author email: MWR@SSI.dk

Have you validated this method, if so, how and what were the results of the validation?​

Method from a coronavirus lab in Denmark

Heat Inactivation

One reported bottle neck is the lysis buffer, but a lab in Denmark who are experts on coronavirus have shown that the virus can be inactivated by heat. (preprint attached, contact details below)

Maiken W. Rosenstierne, Ph.D

Senior Scientist
Virus Research and Development

Department of Virus & Microbiological Special Diagnostics

 

T (direct) +45 3268 3971 | E  MWR@ssi.dk | B  351/207 | W  ssi.dk
Address:  Artillerivej 5 | DK-2300 Copenhagen S

Their email address is MWR@ssi.dk

 

How quickly could this be deployed and what are the dependencies?​

Requires heating method

What is the likely production volume?​

Heating instead of extraction

What are the risks and barriers to using this at scale?​

Requires a heating method

Who are you already partnering with on this?

This is a method developed by a corona virus lab in Denmark

Authors: Anna S. Fomsgaard and Maiken W. Rosenstierne
Affiliations:  Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark
Corresponding author: Maiken W. Rosenstierne
Corresponding author email: MWR@SSI.dk

 

Commenting is closed

Rob King 2 months ago

Status label added: D

Daniel Horton 2 months ago

This method comes from a lab we collaborate with, and we are currently assessing this method alongside others. Heating alone has huge potential advantages, as no extraction buffer is needed. The accuracy compared to traditional extraction and purification based techniques will clearly be lower, and vary depending on the mastermix used, due in part to PCR inhibition. But it all depends what level of sensitivity and specificity is acceptable, and what you are doing with the results.

Kyle Beacham 2 months ago

The idea has been progressed to the next milestone.

Tom Jordan 2 months ago

Status label added: D1

Status label removed: D

caroline austin 2 months ago

what do the status labels mean?

Tom Jordan 2 months ago

Thank you for sharing this with us Caroline. Our champions were keen to share your workflow submission more widely. We have added this page to our weekly Testing Methods bulletin, to encourage labs to consider this in detail, test and scale up.

You can access the weekly bulletins from the COVID-19 resources hub on the RCPath website: https://www.rcpath.org/profession/coronavirus-resource-hub.html

Our team is using the status labels so we can sort and keep track of submissions that we are assessing and sharing more widely.

Kind regards,
Tom Jordan
On behalf of the Testing Methods team

Tom Jordan 2 months ago

The idea has been progressed to the next milestone.

Rocio Martinez-Nunez 2 months ago

We have seen that heat inactivation does not modify the results of the qPCR: https://www.medrxiv.org/content/10.1101/2020....4351v1.full.pdf

Charlotte Cookson 2 months ago

Hi Caroline, we are trying to make contact to see if you would like to join a conversation we are planning for next week. Charlotte

Users tagged:

caroline austin 2 months ago

Hi
what information is needed?
I can join the conversation if it would be of value
However this method was one reported by the lab of
Maiken W. Rosenstierne, Ph.D ,
so would they be a better person to contact? Their email address is MWR@ssi.dk

Maiken W. Rosenstierne, Ph.D
Senior Scientist
Virus Research and Development

Department of Virus & Microbiological Special Diagnostics



T (direct) +45 3268 3971 | E MWR@ssi.dk | B 351/207 | W ssi.dk
Address: Artillerivej 5 | DK-2300 Copenhagen S

Their email address is MWR@ssi.dk

Charlotte Cookson 2 months ago

Status label added: Requires more information

Bev Matthews 1 month ago

Status label added: D3: Catalogue of Resources

Status label removed: More information requested

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