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NWLP - Extraction-Free protocol

by Richard Hale | Apr 13, 2020 | in Catalogue of Resources
D1: Path Lab Bulletin

The team at Charing Cross Hospital have developed a simple protocol using heat inactivation of the virus, followed by direct PCR on the AusDiagnostics High-Plex platform. The initial protocol has used S.P.S. buffer from Serosep, but additional experiments have shown that this is not essential and can be replaced with readily available alternatives.

Have you validated this method, if so, how and what were the results of the validation?​

The method has been validated - please see the attached document. Further data will be available in the next day or two.

How quickly could this be deployed and what are the dependencies?​

There are no obvious barriers to immediate deployment

What is the likely production volume?​

Production volumes should not be an issue as long as the alternatives to S.P.S. are in free supply.

What are the risks and barriers to using this at scale?​

This method has only been tested with the AusDiagnostics two-step MT-PCR process so far. Extraction-free methods are generally less sensitive than protocols that use an extraction step. This has been mitigated by adding additional cycles in Step 1 of the MT-PCR process.

Who are you already partnering with on this?

This work has been carried out at Charing Cross Hospital. 

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Kyle Beacham Apr 14, 2020

Status label added: D

Kyle Beacham Apr 14, 2020

The idea has been progressed to the next milestone.

Bev Matthews Apr 23, 2020

Status label added: D1

Bev Matthews Apr 23, 2020

Status label removed: D

Bev Matthews Apr 25, 2020

Status label added: D

Status label removed: D1: Path Lab Bulletin

Bev Matthews Apr 25, 2020

Hello Richard, this solution has also been submitted https://testingmethods.crowdicity.com/post/3163680. please can you review and confirm whether the reviewers need to consider this as a separate solution please. Thank you Bev

Tom Jordan Apr 30, 2020

Status label added: D1: Path Lab Bulletin

Bev Matthews May 3, 2020

Status label removed: D: Ready to share

Bev Matthews May 3, 2020

Links with https://testingmethods.crowdicity.com/post/3163680

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