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Trizol or equivalent reagent - stable, well tested and good for downstream column prep.

A: Not for current challenges
Closed

Place samples in Trizol reagent for safe storage of RNA and complete inactivation of virus.

Have you validated this method, if so, how and what were the results of the validation?

Yes, for other CAt3 agents. Trizol safely digests all tissue/cells/viruses and will safely keep RNA stable. Is the main reagent for Directzol RNA column (Cambridge Bioscince) prep - best for producing useful RNA from small amounts of sample.

How quickly could this be deployed and what are the dependencies?

Trizol and equivalent reagents contain phenol, which should be handled with care. It is widely available in most labs and stable for transport, but needs to be handled by experienced lab technicians.

What is the likely production volume?

Only require very small amounts to stabilise RNA by denaturing proteins. < 200 µL/sample would be safe.

What are the risks and barriers to using this at scale?

Phenol containing reagents have associated risks and should be handled with proper protection (nitrile gloves) to avoid potential injury from spillage or splashes to lab workers.

Who are you already partnering with on this?

N/A

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Bev Matthews Apr 21, 2020

The idea has been progressed to the next milestone.

Bev Matthews Apr 21, 2020

Status label added: A

Bev Matthews Apr 21, 2020

Hello Pegine, thank you for your submission, which has now been reviewed. Given our urgent priorities we will not be taking your solution forward at this point but please do let us know if additional information become available. Bev

Bev Matthews Apr 21, 2020

The idea has been progressed to the next milestone.

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